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KMID : 1098420050130060217
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Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 6 p.217 ~ p.225
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Genotoxicity and Mutagenicity of the Extracts of Morus alba L.
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Jin Hyou-Ju
Lee Hyeon-Yong
Kim Jong-Dai
Heo Moon-Young
Lee Jin-Ha
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Abstract
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This study was carried out to investigate the genotoxicity in comet and in vitro micronucleus assay and mutagenicity in Ames test of the extracts from leaves and stem of Morus alba L. The samples showed a very weak cytotoxicity on the NIH/3T3 cells by SRB assay. The cell viability of the extracts and fractions from leaves and stems of Morus alba L. was 80% over at 500;{mu}g/ml, and that of the chloroform fractions from leaves and stems showed lower than others. The genotoxicity at 250;{mu}g/ml of 100% EtOH and water extracts on the NIH/3T3 cells in comet assay was about 40% compared to positive control, and most fractions from 100% EtOH extract of the leaves showed stronger genotoxicity than that offractions from the stem. The genotoxicity with S-9 mix in vitro micronucleus assay of the 100% EtOH and water extracts form Morus alba L. did not indicate any significant difference as compared with control group. The cytokinesis-binucleated cells were showed in the hexan, chloroform, ethylacetate and butanol fractions from the extract of the leaves without S-9, and sample with S-9 showed CB cells in the chloroform fraction from the leaves. In the Ames test, the water and 100% ethanol extracts of Morus alba L. did not have a strong mutagenicity in TA98 and TA100, but the fractions of organic solvents of the ethanol extract had 10{sim}26% of mutagenicity on the TA100 strain.
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KEYWORD
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Morus alba L., cytotoxicity, genotoxicity, mutagenicity, comet, micronucleus assay, Ames test
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